Malaria, the first parasitic endemic in the world, still remains a major public health problem, with sub-Saharan Africa accounting for 80% of the 300 millions of malaria cases reported in the world in 2012 [1] . According to World Health Organization (WHO) global estimations in 2012, 1,000,000 deaths occurred and majority of them among children were less than five years old [1] . Acute kidney injury (AKI) is a common complication of severe malaria (SM) in adults, affecting up to 40% of patients [1] [2] . In the absence of appropriate renal replacement therapy (RRT), the case fatality rate approaches 75% [1] [2] . Clinically and pathologically, AKI in adults with SM resembles acute tubular necrosis [3] . In the world, there are limited data on the incidence of AKI in pediatric SM with estimates ranging from 3% to 31% [4] [5] [6] [7] . In Africa, the few existing data estimates AKI in pediatric SM between 23% and 45% [2] [7] . In Togo, there are only two old epidemiological studies which concerned severe pediatric malaria in general [8] . Our study, the first of its kind in Togo, aims to describe clinical, biological and evolutionary profile of AKI due to SM in the pediatric department of Sylvanus Olympio Teaching Hospital of Lome.

This was a retrospective descriptive study that took place from January to December 2012 in the pediatric department of Sylvanus Olympio Teaching Hospital of Lome. Have been included children aged 0 - 15 years admitted for severe malaria with positive thick drop. Included were all records of both sexes, aged 0 - 15 years, admitted for severe malaria with associated or not to signs of kidney damage such as oliguria, anuria, haematuria, hemoglobinuria, facial edema, or edema of the lower limbs. Not included in our study children admitted for severe malaria with a thick negative drop, those admitted in pediatric with positive thick drop without renal failure. Data concerned sociodemographic, clinical, biological, therapeutic and evolutionary were collected. AKI was defined by using the modified RIFLE (Risk Injury Failure Loss End stage) classification for Pediatrics (pRIFLE): estimated creatinine clearance (calculated according to Schwartz formula) decreased by at least 25%, or diuresis < 0.5 ml/kg/h for 8 hours [9] . When the Creatinine is unknown, the decline in the estimated GFR (Glomerular Filtration R ate) was calculated from of a reference value of 100 ml/min/1.73 m² (Table 1).

Statistical software

The Statistical software SPSS 18.0 was used for the analysis of the data. Student t test (two tailed, independent) has been used to find the significance of

eCrCl, estimated creatinine clearance; pRIFLE, pediatric risk, injury, failure, loss and end-stage renal disease.

study parameters on continuous scale between two groups (Inter group analysis) on metric parameters. Chi square/Fisher Exact test has been used to find the significance of study parameters on categorical scale between two or more groups.

During the study period 338 children admitted for severe malaria were included. AKI was diagnosed in 24 children, a prevalence of 7.1% according to pRIFLE classification: RISK in 10 (3%), INJURY in 9 (2.6%) and FAILURE in 5 (1.5%) (Table 2).

The sex ratio was 2.43 with an average age of 8.16 ± 4.2 years (Figure 1).

The rate of self-medication recovered is high (Table 3).

At admission, clinical features were dominated by hemoglobinuria in 87.5%, oliguria, vomiting and fever in 75%. Hypotension was found in 37.5% of the cases (Table 4).

The biological features were dominated by severe anemia (Hb < 6 g/dl) in 79.2% of cases. Hyperkalemia (K > 5.5 mmol/l) was found in 2 cases with a context of anuria in one case (Table 4). All the thick drops were positive to Plasmodium Falciparum. The mean parasitic density was 22,120 trophozoites with extremes of 190 and 98,810 parasites/mm³. For treatment, 25% of patients received quinine treatment before admission. During hospitalization, 58.3% of children needed blood transfusion. Malaria was treated primarily with artemether in 83.3% of the cases (Table 5).

Dialysis was indicated in 2 cases for menacing hyperkalaemia, but was not done because of lack of financial means. In one case, hyperkalaemia was succefully treated with symptomatic measures (insulin in glucose serum, furosemide, bicarbonate serum). In the second case, these measures not allowed to normalize kalaemia because of anuria, and finally death occurred. In all other cases, renal function was recovered in 91.6%. Only one child had kidney function below 30 ml/min/1.73 m² after 3 weeks. H was referred to nephrologist for follow-up (Figure 2).

Malaria was treated effectively in all cases attested by a negative thick dropscontrol after an average stay of 16 ± 4.4 days.

eCrCl, estimated creatinine clearance; pRIFLE, pediatric risk, injury, failure, loss and end-stage renal disease.

ASAT: aspartate aminotransferase; ALAT: Alanine aminotransferase.

In endemic areas of Malaria, AKI is a common complication, but there are limited data on the incidence of AKI in pediatric SM with estimates ranging from 3% to 31% [4] [5] [6] [7] [10] . In Togo, our study is the first which talk about this item. The prevalence of AKI due to severe malaria is 7.1% in our study, lower than the 23.6% found in the Congo [7] or the 30.4% found in India [11] . This difference would probably be related to the retrospective nature of our study. Our prevalence is nevertheless more important than that of several works of the 2000s [8] [12] [13] , suggesting that the association of AKI with malaria would be a new factor. This hypothesis is supported in several studies which prove the resurgence in endemic area of bilious fever hemoglobinuria which was considered in the years 1950-1970 erroneously as only the disease of nonimmune subjects to malaria [7] [14] [15] [16] . The average age of our patients was 8 years, similar to that reported in the study by Bodi et al. [15] . AKI is predominant in our study by the early stages of the RIFLE classification (R and I stages: 19 of 24 patients, 79.2%), which shows a large part of functional renal failure in pathophysiology of AKI during malaria. Moreover, hypotension was present in 37% of cases, vomiting in 75% and severe anemia in 79%. In the literature, hemoglobinuria, which prevalence remains high in studies [7] [13] [15] , including our study (87%), is the main pathogenesis of acute renal failure during malaria. This hemoglobinuria resulting from intravascular haemolysis is not the only mechanism of AKI due to malaria. It resulting from many factors such as: cytoadherence and erythrocyte sequestration with intravascular coagulation, responsible for hypo-perfusion; dehydration and hypovolemia related to fever, profuse sweating, lack of water supply and digestive disorder, also leading to renal hypo-perfusion; Activation of monocytes with release of cytokines, free radicals and immunoglobulins. These phenomena contribute to the establishment of acute tubular necrosis in malaria [7] . In our study, nearly one quartz of patients had been treated with quinine (25%) and analgesic (20%), which could be another factor of AKI. The hemolyzing effect of quinine is known in particular hemoglobinopathies [7] [14] [15] [17] [18] . The majority of our patients have been treated with artemether with total effectiveness. However, 2 patients presented hyperkalaemia requiring emergency dialysis, but not realized due to lack of financial means leading to the death of one patient. The problem of lack of care in emergency dialysis is recurring in Togo where until now there is only one hemodialysis center for the whole country [19] . Patients pay for their hemodialysis sessions themselves. There is only one adult nephrologist per 7 million inhabitants [19] . Peritoneal dialysis, normally adapted to children, is not available. The mortality in our study is 4.16%, different from the results of Ivoiry Coast, Nigeria and Congo where it is above 10% [7] [14] [20] . The average hospital stay is 16 + 4.4 days, similar to the results of Kunuanunua [7] . Classic duration of acute tubular necrosis evolution is the explanation. Of the 24 patients collected, 95% fully recovered renal function. A single patient who has not fully recovered will gradually progress to chronic renal failure and will be an additional burden on the already dying health system. The weakness of study is it’s retrospective nature. Obviously the size of our sample does not allow us to make relevant statistical tests.

Acute renal failure often secondary to tubular necrosis may be the dominant clinical feature of severe malaria. Death may occur; its prognosis depends on early diagnosis and the prompt introduction of adequate treatment. As always, malaria prevention would be the best way to avoid it. In Togo the setting up of a structure adapted to the management of acute kidney failure in general and especially adapted to pediatrics will improve the prognosis of this pathology of which our study shows only the part emerged from the iceberg because of its retrospective and monocentric character.

All data generated or analyzed during this study are included in this published article.

No particular acknowledgment.

Not applicable.

No funding was received.

All authors have contributed to a published study to conception and design, acquisition of data, analysis and interpretation of data, drafting the manuscript, revising it critically for important intellectual content and have given final approval of the version to be published.

In the world, there are limited data on the incidence of Acute Kidney Injury (AKI) in pediatric Severe Malaria (SM). In Africa, the existing data is very limited.

Our study, the first of its kind in Togo, aims to describe clinical, biological and evolutionary profile of AKI due to SM in the pediatric department of Sylvanus Olympio Teaching Hospital of Lome. Our paper discusses the possibility to develop peritoneal dialysis in this country.

The authors of this manuscript have no conflicts of interest to disclose as described by the OJNEPH.

Sabi, K.A., Kaza, B.N.K., Amekoudi, E.Y.M., Tia, W.M., Vigan, J., Ibrahim, H., Mahamat, G.A., Amouzou-Glikpa, S. and Djadou, E. (2018) Acute Kidney Injury during Malaria in Togolese Children. Open Journal of Nephrology, 8, 85-93. https://doi.org/10.4236/ojneph.2018.84010

AKI: Acute Kidney Injury; SM: Severe Malaria; RIFLE: Risk Injury Failure Loss End stage.