<?xml version="1.0" encoding="UTF-8"?><!DOCTYPE article  PUBLIC "-//NLM//DTD Journal Publishing DTD v3.0 20080202//EN" "http://dtd.nlm.nih.gov/publishing/3.0/journalpublishing3.dtd"><article xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink" dtd-version="3.0" xml:lang="en" article-type="research article"><front><journal-meta><journal-id journal-id-type="publisher-id">AJPS</journal-id><journal-title-group><journal-title>American Journal of Plant Sciences</journal-title></journal-title-group><issn pub-type="epub">2158-2742</issn><publisher><publisher-name>Scientific Research Publishing</publisher-name></publisher></journal-meta><article-meta><article-id pub-id-type="doi">10.4236/ajps.2015.62041</article-id><article-id pub-id-type="publisher-id">AJPS-54081</article-id><article-categories><subj-group subj-group-type="heading"><subject>Articles</subject></subj-group><subj-group subj-group-type="Discipline-v2"><subject>Biomedical&amp;Life Sciences</subject></subj-group></article-categories><title-group><article-title>
 
 
  In-Vitro Efficacy of Certain Essential Oils and Plant Extracts against Three Major Pathogens of Jatropha curcas L.
 
</article-title></title-group><contrib-group><contrib contrib-type="author" xlink:type="simple"><name name-style="western"><surname>urnajyoti</surname><given-names>Deka Bhuyan</given-names></name><xref ref-type="aff" rid="aff1"><sup>1</sup></xref></contrib><contrib contrib-type="author" xlink:type="simple"><name name-style="western"><surname>Phatik</surname><given-names>Tamuli</given-names></name><xref ref-type="aff" rid="aff2"><sup>2</sup></xref><xref ref-type="corresp" rid="cor1"><sup>*</sup></xref></contrib><contrib contrib-type="author" xlink:type="simple"><name name-style="western"><surname>Paran</surname><given-names>Boruah</given-names></name><xref ref-type="aff" rid="aff1"><sup>1</sup></xref></contrib></contrib-group><aff id="aff1"><addr-line>Division of Medicine, India Aromatic and Economic Plants, North East Institute of Science &amp;amp; Technology (CSIR), Jorhat, India</addr-line></aff><aff id="aff2"><addr-line>Microbiology and Plant Pathology Lab, Department of Botany, Darrang College, Tezpur, India</addr-line></aff><author-notes><corresp id="cor1">* E-mail:<email>tamulip@yahoo.com(PT)</email>;</corresp></author-notes><pub-date pub-type="epub"><day>30</day><month>01</month><year>2015</year></pub-date><volume>06</volume><issue>02</issue><fpage>362</fpage><lpage>365</lpage><history><date date-type="received"><day>9</day>	<month>December</month>	<year>2014</year></date><date date-type="rev-recd"><day>accepted</day>	<month>10</month>	<year>February</year>	</date><date date-type="accepted"><day>15</day>	<month>February</month>	<year>2015</year></date></history><permissions><copyright-statement>&#169; Copyright  2014 by authors and Scientific Research Publishing Inc. </copyright-statement><copyright-year>2014</copyright-year><license><license-p>This work is licensed under the Creative Commons Attribution International License (CC BY). http://creativecommons.org/licenses/by/4.0/</license-p></license></permissions><abstract><p>
 
 
  Antifungal activity of plant extracts and essential oils of six different plant species was tested against three pathogenic fungi, viz., Alternaria alternata, Colletotrichum gloeosporioides and Fusarium moniliforme isolated from Jatropha curcas L. using Poison Food Technique. All the samples tested were found effective in-vitro. More than 60% inhibition of growth of individual fungal species was observed at 100 ppm. Maximum inhibition was observed at concentration of 1000 ppm. However, among the essential oils tested Cinnamomum impressinervium exhibited the strongest activity (80%) in the case of Colletotrichum gloeosporioides and Alternaria alternate and 78.6% in the case of Fusarium oxysporum at concentration of 1000 ppm followed by Cinnamomum tamala, Cymbopogon jwarancusa and Cymbopogon citratus respectively. Among the plant extracts tested, Catharanthus roseus exhibited stronger activity in comparison to Tithonia diversifolia. Inhibition percentage of all the essential oils and plant extracts increased with the increase in concentration.
 
</p></abstract><kwd-group><kwd>Antifungal Activity</kwd><kwd> Alternaria alternate</kwd><kwd> Colletotrichum gloeosporioides</kwd><kwd> Fusarium moniliforme</kwd><kwd> Jatropha curcas</kwd></kwd-group></article-meta></front><body><sec id="s1"><title>1. Introduction</title><p>Antifungal activity of some isolated principles from plant extracts may be more effective than some commercial synthetic fungicides. The presence of naturally occurring substances in plants with anti microbial properties have been recognized and tested against a wide range of pathogenic microbes [<xref ref-type="bibr" rid="scirp.54081-ref1">1</xref>] . With the increase of interest in antibiotics plants as a source of potential antimicrobial substances are receiving considerable attention throughout the world. Recently many aqueous plant extracts have been shown to have inhibitory action against some plant as well as human pathogenic microbes. Nowadays some synthetic as well as semi-synthetic antimicrobial agents have been developing, among which very few have broad spectrum activity and most of them are environmentally hazardous in nature. The extensive use of agrochemicals especially fungicides, resulted more carcinogenic risk than other pesticides which may give rise to undesirable biological effects on animals and human beings [<xref ref-type="bibr" rid="scirp.54081-ref2">2</xref>] .</p><p>Jatropha curcas is a multipurpose shrub or small tree belonging to the family of Euphorbiaceae [<xref ref-type="bibr" rid="scirp.54081-ref3">3</xref>] . This plant has received increasing interest as a bio-diesel plant since the beginning of the 21st century. The seeds also contain viscous non-edible oil (about 48%) which besides being a source of bio-diesel, can also be used for manufacturing various useful products, candles etc. This valuable plant species have been attacked by various fungal pathogens causing severe loss. Present investigation was carried out to control three major fungal pathogens, viz., Alternaria alternata (Fr.) Keissl., Colletotrichum gloeosporioides (Penz.) Penz. &amp; Sacc. and Fusarium moniliforme Sheld., isolated from diseased plant parts of Jatropha curcas. C. gloeosporioides is responsible for anthracnose in Jatropha curcas fruit. The disease results in fruit rot and fruit drop and ultimately lead to the yield loss. F. moniliforme caused fruit decay. Alternaria leaf spot disease caused premature defoliation.</p><p>Investigations have been carried out with an aim to control the diseases of Jatropha curcas using essential oils and plant extracts of the some selected plant species. Cinnamomum impressinervium Meissn. (Family―Lau- raceae) is a small to medium sized evergreen tree found in evergreen forest of northern state including Sikkim at an altitude between 220 - 1200 m. Leaves are aromatic possessing a strong spicy odour and used as spice in the region. Leaf oil of this species contains high amount of eugenol (88.3%) [<xref ref-type="bibr" rid="scirp.54081-ref4">4</xref>] [<xref ref-type="bibr" rid="scirp.54081-ref5">5</xref>] . Cinnamomum tamala Nees. (Family―Lauraceae), is a medium sized tree distributed in tropical and subtropical Himalayas at the altitudes of 1000 - 2400 m. In India, the tree is cultivated commercially in certain parts of the country for leaf production and essential oils. Leaves and oils are used in flavouring foods, beverages, in perfumery and pharmaceutical industries [<xref ref-type="bibr" rid="scirp.54081-ref6">6</xref>] . Cymbopogon jwarancusa (Jones) schult. (Family―Poaceae) is one of the cultivars developed by CSIR-NEIST, Jorhat (Assam), India. Major constituent of the oil of this species is piperitone (83%) [<xref ref-type="bibr" rid="scirp.54081-ref7">7</xref>] . Cymbopogon citratus (DC.) Stapf. (Family―Poaceae), is an important aromatic plant, a source of essential oil and is widely used as a component of ethno-pharmaceuticals in tropical and subtropical countries. The aerial parts of the plant are widely used in folk medicine to treat various health problems [<xref ref-type="bibr" rid="scirp.54081-ref8">8</xref>] . The essential oil of Cymbopogon citratus has antifungal and insecticidal activities [<xref ref-type="bibr" rid="scirp.54081-ref9">9</xref>] . Tithonia diversifolia Hemsley. Gray (Family―Asteraceae) is widely distributed throughout the humid and sub-humid tropics in central and south America, Africa and all Asian countries. Extracts from the leaf of T. diversifolia contain sesquiterpene lactones e.g. tagitinin which possess insecticidal properties [<xref ref-type="bibr" rid="scirp.54081-ref10">10</xref>] . Catharanthus roseus (L.) G. Don (Family―Apocynaceae) is a medicinal plant grown throughout India and found as an escape in waste places and sandy tracts. The aerial part of the plant contains about 130 different alkaloids from which well-known high value secondary metabolites vincristine and vinblastine are used in chemotherapy to treat diverse cancers, while ajmalicine and serpentine are prescribed for hypertension.</p></sec><sec id="s2"><title>2. Materials and Methods</title><sec id="s2_1"><title>2.1. Plant Materials and Test Pathogens</title><p>The leaves of selected plant species were collected from experimental farm of CSIR-North East Institute of Science and Technology, Jorhat (Assam), India. These were washed 2 - 3 times in tap water and air dried at room temperature (25˚C - 30˚C). The fungal pathogens were isolated from the diseased parts of Jatropha curcas, characterized and were identified through high power microscopy.</p></sec><sec id="s2_2"><title>2.2. Preparation of the Extracts and Essential Oils</title><p>The dried leaves (Catharanthus roseus and Tithonia diversifolia) were ground into powder form, sieved and packaged into polyethylene bags until when needed. 50 g sample of powdered dried leaves were weighed and extracted in Soxhlet extractor with ethanol at 40˚C - 60˚C. Essential oils were obtained by subjecting the fresh leaves to hydrodistillation in clevengers apparatus for 4 hours. Oils were collected in glass vials after removing water traces by sodium sulphate and stored at 4˚C. The extracts and essential oil obtained were assayed against the test organisms to determine the antifungal properties.</p></sec><sec id="s2_3"><title>2.3. Determination of Antifungal Activity</title><p>The retrieved extract was tested for its antifungal activity using Poisoned Food Technique [<xref ref-type="bibr" rid="scirp.54081-ref11">11</xref>] . 20 ml of potato dextrose agar was poured into sterilized petri plates and measured amount of oil sample/extracts were added, allowed them to mix homogeneously and to be solidified. Control growth medium contained equivalent amounts solvent. Fungal disks of 5 mm in diameter from an 8-day-old pure culture were placed in the center of the Petri dish containing medium under aseptic condition, incubated at 27˚C &#177; 1˚C for 7 days. The experiments were carried out in three replicates per treatment. Growth of each fungal species was observed and recorded after one week of incubation. Percent inhibition was computed after comparison with the control. Percent inhibition of mycelial growth was calculated using the following formula [<xref ref-type="bibr" rid="scirp.54081-ref12">12</xref>] .</p><disp-formula id="scirp.54081-formula882"><graphic  xlink:href="http://html.scirp.org/file/12-2601883x6.png"  xlink:type="simple"/></disp-formula></sec></sec><sec id="s3"><title>3. Results and Discussion</title><p>All the samples showed antifungal activity in-vitro. However, among the essential oils tested Cinnamomum impressinervium exhibited strongest activity at concentration of 1000 ppm (80.0, 78.6 and 80.0 against C. gloeosporioides, F. moniliforme and A. alternate respectively) followed by C. tamala (77.3, 76.8, 78.0), Cymbopogon jwarancusa (73.4, 71.4, 74.0) and C. citratus (67.0, 66.5, 68.6) (<xref ref-type="table" rid="table1">Table 1</xref>). Catharanthus roseus exhibited stronger activity (72.5, 70.1, 73.0 against C. gloeosporioides, F. moniliforme and A. alternata respectively) in comparison to Tithonia diversifolia (68.6, 66.5, 67.0) (<xref ref-type="table" rid="table1">Table 1</xref>). It was observed that inhibition percentage of all the essential oils as well as plant extracts increased with the increase in concentration. All the tested plant spe- cies were found to have good antifungal activity.</p><table-wrap id="table1" ><label><xref ref-type="table" rid="table1">Table 1</xref></label><caption><title> The inhibitory effects of the samples on mycelial growth of test fungi 7 days after inoculation (percent inhibition)</title></caption><table><tbody><thead><tr><th align="center" valign="middle"  rowspan="2"  >Plant extracts/ Essential oils</th><th align="center" valign="middle"  rowspan="2"  >Test fungi</th><th align="center" valign="middle"  colspan="11"  >Concentrations (ppm)</th></tr></thead><tr><td align="center" valign="middle"  colspan="2"  >100</td><td align="center" valign="middle"  colspan="2"  >200</td><td align="center" valign="middle"  colspan="2"  >400</td><td align="center" valign="middle"  colspan="2"  >600</td><td align="center" valign="middle"  colspan="2"  >800</td><td align="center" valign="middle" >1000</td></tr><tr><td align="center" valign="middle" >Cinnamomum impressinervium</td><td align="center" valign="middle" >Cg Fm Aa</td><td align="center" valign="middle" >68.0 67.8 68.5</td><td align="center" valign="middle"  colspan="2"  >70.2 69.9 70.9</td><td align="center" valign="middle"  colspan="2"  >72.5 72.0 73.3</td><td align="center" valign="middle"  colspan="2"  >74.8 74.2 75.6</td><td align="center" valign="middle"  colspan="2"  >77.5 76.8 77.8</td><td align="center" valign="middle"  colspan="2"  >80.0 78.6 80.0</td></tr><tr><td align="center" valign="middle" >Cinnamomum tamala</td><td align="center" valign="middle" >Cg Fm Aa</td><td align="center" valign="middle" >66.8 66.0 67.0</td><td align="center" valign="middle"  colspan="2"  >68.9 68.4 69.0</td><td align="center" valign="middle"  colspan="2"  >71.2 70.8 71.7</td><td align="center" valign="middle"  colspan="2"  >73.5 73.0 73.9</td><td align="center" valign="middle"  colspan="2"  >75.8 75.0 76.0</td><td align="center" valign="middle"  colspan="2"  >77.3 76.8 78.0</td></tr><tr><td align="center" valign="middle" >Cymbopogon citratus</td><td align="center" valign="middle" >Cg Fm Aa</td><td align="center" valign="middle" >62.1 61.8 63.2</td><td align="center" valign="middle"  colspan="2"  >63.0 62.8 63.9</td><td align="center" valign="middle"  colspan="2"  >64.5 64.0 64.8</td><td align="center" valign="middle"  colspan="2"  >66.0 65.2 66.6</td><td align="center" valign="middle"  colspan="2"  >66.9 66.1 67.1</td><td align="center" valign="middle"  colspan="2"  >67.0 66.5 68.6</td></tr><tr><td align="center" valign="middle" >Cymbopogon jwarancusa</td><td align="center" valign="middle" >Cg Fm Aa</td><td align="center" valign="middle" >64.0 62.2 65.5</td><td align="center" valign="middle"  colspan="2"  >67.0 65.5 68.0</td><td align="center" valign="middle"  colspan="2"  >68.6 67.0 69.6</td><td align="center" valign="middle"  colspan="2"  >70.2 68.5 71.0</td><td align="center" valign="middle"  colspan="2"  >71.8 69.5 72.4</td><td align="center" valign="middle"  colspan="2"  >73.4 71.4 74.0</td></tr><tr><td align="center" valign="middle" >Catharanthus roseus</td><td align="center" valign="middle" >Cg Fm Aa</td><td align="center" valign="middle" >62.0 61.3 64.4</td><td align="center" valign="middle"  colspan="2"  >64.1 62.8 66.0</td><td align="center" valign="middle"  colspan="2"  >66.6 64.5 67.2</td><td align="center" valign="middle"  colspan="2"  >68.6 66.5 68.2</td><td align="center" valign="middle"  colspan="2"  >70.5 68.3 71.8</td><td align="center" valign="middle"  colspan="2"  >72.5 70.1 73.0</td></tr><tr><td align="center" valign="middle" >Tithonia diversi Fmlia</td><td align="center" valign="middle" >Cg Fm Aa</td><td align="center" valign="middle" >62.0 61.5 62.8</td><td align="center" valign="middle"  colspan="2"  >63.3 62.4 63.5</td><td align="center" valign="middle"  colspan="2"  >65.1 63.0 64.6</td><td align="center" valign="middle"  colspan="2"  >66.0 64.4 65.5</td><td align="center" valign="middle"  colspan="2"  >67.5 65.5 66.1</td><td align="center" valign="middle"  colspan="2"  >68.6 66.5 67.0</td></tr><tr><td align="center" valign="middle" >Control (Solvent)</td><td align="center" valign="middle"  colspan="12"  >Cg fungal growth 99.8% Fm 100% Aa 99.5%</td></tr><tr><td align="center" valign="middle" >Control (Sterile distilled water)</td><td align="center" valign="middle"  colspan="12"  >Cg fungal growth 100% Fm 100% Aa 100%</td></tr><tr><td align="center" valign="middle" ></td><td align="center" valign="middle" ></td><td align="center" valign="middle" ></td><td align="center" valign="middle" ></td><td align="center" valign="middle" ></td><td align="center" valign="middle" ></td><td align="center" valign="middle" ></td><td align="center" valign="middle" ></td><td align="center" valign="middle" ></td><td align="center" valign="middle" ></td><td align="center" valign="middle" ></td><td align="center" valign="middle" ></td><td align="center" valign="middle" ></td></tr></tbody></table></table-wrap><p>In present investigation all the tested plants found to be effective against C. gloeosporioides, F. moniliforme and A. alternata. They too showed increasing inhibitory effect on the fungal growth with higher concentration</p><p>compared to control [<xref ref-type="bibr" rid="scirp.54081-ref13">13</xref>] while studying the efficacy of 16 plant extracts in controlling the leaf spot in ginger caused by Phyllostica zingiberis found that growth inhibition of fungi increased with increasing concentration of extracts. [<xref ref-type="bibr" rid="scirp.54081-ref14">14</xref>] screened 12 angiospermic plant extracts in and around the same locality of Sonitpur district. Out of these, 6 plant extracts Mikania scandence, Eupatorium odoratum, Cassia sophera, Leucus plunketii, Occimum basilicum and Clitoria ternate were found effective in total inhibition of mycelia growth of S. sclerotiorum.</p><p>The inhibition of the growth of the pathogenic fungi is due to the active ingradients predominantly found in the plant [<xref ref-type="bibr" rid="scirp.54081-ref15">15</xref>] . The present investigations are in line with the investigations carried out by other workers [<xref ref-type="bibr" rid="scirp.54081-ref16">16</xref>] who infer that leaf extracts in general have great potentiallity in the control of fungal diseases in commercially important crop plants. It may be concluded that keeping aside the environmentally hazardous commercial fungicides, these leaf extracts could be suitable substitute for controlling fungal pathogens. It may be concluded that keeping aside the environmentally hazardous commercial fungicides, these leaf extracts and essential oils could be a suitable substitute for controlling the fungal pathogens.</p></sec><sec id="s4"><title>NOTES</title></sec></body><back><ref-list><title>References</title><ref id="scirp.54081-ref1"><label>1</label><mixed-citation publication-type="other" xlink:type="simple">Tamuli, P., Das, J. and Boruah, P. (2014) Antifungal Activity of Polygonum Hydropiper and Solanum Melongena against Plant Pathogenic Fungi. Plant Archives, 14, 15-17.</mixed-citation></ref><ref id="scirp.54081-ref2"><label>2</label><mixed-citation publication-type="other" xlink:type="simple">Osman, K.A. and Abdulrahman, H.T. (2003) Risk Assessment of Pesticides to Human and the Environment. Saudi Journal of Biological Science, 10, 81-106.</mixed-citation></ref><ref id="scirp.54081-ref3"><label>3</label><mixed-citation publication-type="other" xlink:type="simple">Ye, M., Li, C.Y., Francis, G. and Harinder, P.S. (2009) Current Situation and Prospects of Jatropha curcas as a Multipurpose Tree in China. Agroforestry Systems, 76, 487-497. http://dx.doi.org/10.1007/s10457-009-9226-x</mixed-citation></ref><ref id="scirp.54081-ref4"><label>4</label><mixed-citation publication-type="other" xlink:type="simple">Nath, S.C. and Baruah, A.K.S. (1994) Eugenol as the Major Component of the Leaf Oil of Cinnamomum impressinervium Meissn. Journal of Essential Oil Research, 6, 211-212. http://dx.doi.org/10.1080/10412905.1994.9698360</mixed-citation></ref><ref id="scirp.54081-ref5"><label>5</label><mixed-citation publication-type="other" xlink:type="simple">Nath, S.C., Baruah, A. and Hazarika, A.K. (1999) Essential Oil of Leaves of Cinnamomum schaeffer Member. Indian Perfumer, 43, 182-190.</mixed-citation></ref><ref id="scirp.54081-ref6"><label>6</label><mixed-citation publication-type="other" xlink:type="simple">Mohan, M.S., Haider, Z., Sharma, A., Seth, R. and Sharma, M. (2012) Antimicrobial Activity and Composition of the Volatiles of Cinnamomum tamala Nees. and Murraya koenigii (L.) Spreng. Asian Pacific Journal of Tropical Disease, 2, S324-S327.</mixed-citation></ref><ref id="scirp.54081-ref7"><label>7</label><mixed-citation publication-type="other" xlink:type="simple">Annonymous (1994) Chemical Abstract, 121, Article ID: 2263383.</mixed-citation></ref><ref id="scirp.54081-ref8"><label>8</label><mixed-citation publication-type="other" xlink:type="simple">Olaniyi, A.A., Sofowora, E.A. and Oguntimehin, B.O. (1975) Phytochemical Investigation of Some Nigerian Plants Used against Fevers. II. Cymbopogon citratus. Planta Medica, 28, 186-189. http://dx.doi.org/10.1055/s-0028-1097851</mixed-citation></ref><ref id="scirp.54081-ref9"><label>9</label><mixed-citation publication-type="other" xlink:type="simple">Irkin, R. and Korukluoglu, M. (2009) Effectiveness of Cymbopogo citratus L. Essential Oil to Inhibit the Growth of Some Filamentous Fungi and Yeasts. Journal of Medicinal Food, 12, 193-197.  
http://dx.doi.org/10.1089/jmf.2008.0108</mixed-citation></ref><ref id="scirp.54081-ref10"><label>10</label><mixed-citation publication-type="other" xlink:type="simple">Liasu, M.O. and Achakzai, A.K.K. (2007) Influence of Tithonia diversifolia Leaf Mulch and Fertilizer Application on Growth and Yield of Potted Tomato Plants. American-Eurasian Journal of Agric. and Environmental Science, 2, 335-340.</mixed-citation></ref><ref id="scirp.54081-ref11"><label>11</label><mixed-citation publication-type="other" xlink:type="simple">Grover, R.K. and Moore, J.D. (1962) Toximetric Studies of Fungicides against the Brown Rot Organisms, Sclerotinia fructicola and S. laxa. Phytopathology, 52, 876-879.</mixed-citation></ref><ref id="scirp.54081-ref12"><label>12</label><mixed-citation publication-type="other" xlink:type="simple">Ogbebor, N.O., Adekunle, A.T. and Enobakhare, D.A. (2007) Inhibition of Colletotrichum gloeosporioides (Penz) Sac. Causal Organism of Rubber (Hevea brasiliensis Muell. Arg.) Leaf Spot Using Plant Extract. African Journal of Biotechnology, 6, 213-218.</mixed-citation></ref><ref id="scirp.54081-ref13"><label>13</label><mixed-citation publication-type="other" xlink:type="simple">Sood, R. and Dohroo, N.P. (2003) Efficacy of Botanicals in Vitro against Phyllostictus zingiberis Causing Leaf Spot of Ginger. Plant Disease Research (Ludhiana), 18, 174-175.</mixed-citation></ref><ref id="scirp.54081-ref14"><label>14</label><mixed-citation publication-type="other" xlink:type="simple">Das, J. and Das, T.K. (2006) Botanicals to Control Phytopathogenic Fungus Sclerotinia scelerotiorum (Lib) De-Bary. Geobios, 33, 203-205.</mixed-citation></ref><ref id="scirp.54081-ref15"><label>15</label><mixed-citation publication-type="other" xlink:type="simple">Shetty, S.A., Prakash, H.S. and Shetty, H.S. (1989) Efficacy of Certain Plant Extract against Seed-Borne Infection of Trichoconiella padwickii in Paddy (Oryza sativa). Canadian Journal of Botany, 67, 1956-1958.  
http://dx.doi.org/10.1139/b89-248</mixed-citation></ref><ref id="scirp.54081-ref16"><label>16</label><mixed-citation publication-type="journal" xlink:type="simple"><name name-style="western"><surname>Sarvamangala</surname><given-names> H.S.</given-names></name>,<name name-style="western"><surname> Goviandaiah and Datta</surname><given-names> R.K. </given-names></name>,<etal>et al</etal>. (<year>1993</year>)<article-title>Evaluation of Plant Extracts for the Control of Fungal Disease of Mulberry</article-title><source> Indian Phytopathology</source><volume> 46</volume>,<fpage> 398</fpage>-<lpage>401</lpage>.<pub-id pub-id-type="doi"></pub-id></mixed-citation></ref></ref-list></back></article>